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Diagnostics: HPLC Complete Kits
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ClinRep® Diagnostics
Parameters
of clinical chemistry may be categorized according
to their clinical purpose: for
Diagnostic parameters are endogenous
substances that show a specific relationship to altered metabolic
processes.
Therefore they are used for the diagnosis of specific diseases and
affections.
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Alcoholism
CDT (Carbohydrate-Deficient Tansferrin) in Serum
CDT (Carbohydrate-Deficient Transferrin) in Serum –
On-Line Analysis |
Tumor
Diseases (Biogenic Amines)
Catecholamines in Plasma
Catecholamines in Urine
Catecholamines in Urine – On-Line Analysis with Fluorescence Detection
Metanephrines in
Urine
Serotonin in
Plasma
Serotonin in Urine
VMA, HVA, 5-HIAA in
Urine
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Diabetes,
Hemoglobin Testing
Hemoglobin A1c (HbA1c) in Whole Blood with DiamatTM
Hemoglobin A1c (HbA1c) in Whole Blood with VariantTM |
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Metabolic
Diseases (Biogenic Amines)
5-Aminolevulinic Acid / Porphobilinogen in Urine
Homocysteine in Plasma
Hydroxyproline in Urine
Phenylalanine (Tyr, Phe, Trp) in Whole Blood, Plasma and
from
Filterspots
Porphyrins Differentiated in Urine
Total Porphyrins in Urine |
Oxidative
Stress
Malondialdehyde
in Plasma/Serum
Vitamin Status
Vitamins A and E in Plasma
Vitamin B1 in Whole Blood
Vitamin B2 in Whole Blood
Vitamin B6 in Plasma / Whole Blood
Vitamins B1, B2 in Whole Blood and Vitamin B6 in Plasma / Whole Blood (Combikit)
Vitamin C in Plasma
25-OH-Vitamin D3 in Plasma/Serum |
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ClinRep® Therapeutic Drug Monitoring
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ClinTox®
Human Biomonitoring
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Parameters for TDM are drugs (and drug metabolites)
which are measured in order to control the appropriate clinical
medication with the respective drug. |
Parameters for HBM serve for
the assessment of an occupational
or environmental
related exposure towards
harmful substances.
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ClinRep® and ClinTox® complete kits are designed
for a fast, simple and accurate analysis of
these parameters under routine conditions.
The kits
contain all reagents required for sample preparation, system
testing, calibration and analysis. |
Antiarrhythmics
Amiodarone in Plasma
Antiasthmatics
Theophylline, Theobromine and Caffeine
in Plasma
Antiepileptic Drugs
Antiepileptics in Serum
Lamotrigine and Sulthiame in Plasma
Antipsychiotics
Atypical Neuroleptics (Olanzapine, Clozapine, Quetiapine)
in Plasma/Serum - On-Line Analysis
Immunosuppressants
Cyclosporine A in Whole Blood
Mycophenolic Acid in Plasma |
Benzene and Benzene Derivatives
ortho-Cresol and Phenol in Urine
Hippuric Acid and Methylhippuric Acids in Urine
t,t-Muconic Acid in Urine
Polycyclic Aromatic Hydrocarbons (PAH)
1-Hydroxypyrene in Urine – On-Line Analysis
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Alcoholism
CDT
(Carbohydrate-Deficient Tansferrin) in
Serum
Quantification of
all transferrin isoforms
Detection of genetic variants
Screening for CDG syndromes
CDT (carbohydrate-deficient transferrin) is the Native
sample, pathological range most
specific parameter for chronic alcohol abuse. The present reagent kit
can be applied for routine diagnostics
as well as for confirmation analysis in case
of unplausible diagnostic findings. The test allows
the identification of genetic variants and can be
used for the screening of CDG syndromes. The present application may
also be performed as a fully automated
method with on-line sample
preparation.
Test Data
Lower detection limit: 0.4 %*
Lower determination limit: 0.6 %*
Intra-assay precision: 4.2 %
Interassay precision: 4.3 % *depends
on the amount on total transferrin
HPLC Parameters
Pump: ternary gradient pump, Flow
rates: 1.2, 2.0 ml/min
Injection volume: 400 - 500 μl, Injection
interval: 20 min
UV/VIS-Detector: 460 nm
HPLC-Thermostat: 40 °C
Sample Preparation
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Alcoholism
CDT
(Carbohydrate-Deficient Tansferrin) in
Serum –
On-Line
Fully automated
method
Run time: 9.5 min
The present on-line test kit is designed as a fast
Native sample, pathological range (injection
interval: 9.5 min) and fully automated method
for CDT analysis. A manual sample preparation is
not required, but is performed on-line within the
HPLC system. The pretreatment step (see below)
serves for the iron saturation of the samples and
is performed automatically by a barcode sampling system.
Test Data
Lower detection limit: 0.4 %*
Lower determination limit: 0.6 %*
Intraassay precision: 4.5 %
Interassay precision: 3.9 %
*depends on the amount on total transferrin
HPLC Parameters
Pump 1: binary gradient pump, flow
rate: 1.4 ml/min
Pump 2: isocratic pump, flow rate:
0.4 ml/min
Injection volume: 400 - 500 μl
Injection interval: 9.5 min
UV/VIS-Detector: 460 nm
HPLC-Thermostat: 40 °C
Sample Pretreatment
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Diabetes,
Hemoglobin Testing
Hemoglobin
A1c (HbA1c) in Whole Blood
(for DiamatTM
and Modulary HPLC)
Reliable
and
cost-efficient analysis
“Gold standard”
for HbA1c analysis
Column lifetime: > 1000 analyses
Diabetes mellitus is a
chronic disease that is associated with abnormally high blood glucose
levels. The determination of HbA1c is an important diagnostic tool for
monitoring the efficiency of dietary control and therapy during
treatment of diabetes mellitus. Due to the excellent specificity and
reproducibility, HPLC is still considered as the “gold standard”
method for the adjustment of the blood glucose concentration in diabetes
patients. The HbA1c values, obtained with the present method, are in
accordance with the value level of the DCCT (Diabetes Control and
Comparison Trial). The kit can be applied on the BioRad analyzer
DiamatTM or any conventional modular HPLC system and features in
robustness and cost efficiency (column lifetime > 1000 analyses).
Test Data
Linearity: The test is linear
over the whole range of physiologically
occurring HbA1c values (approx. 3 -16 % HbA1c).
Intraassay precision: 1 - 2 %
Interassay precision: 2 - 3 %
Dependence on the amount of total hemoglobin:
The HbA1c value is independent on
the amount of total
hemoglobin in a range from 6 - 24 g/dl total hemoglobin.
HPLC Parameters for
Modular HPLC Systems / for DiamatTM
Pump: Ternary Gradient Pump /
Ternary Gradient Pump
Flow rate: 1.5 ml/min / 1.1, 1.5 or 1.8 ml/min
Injection volume: 20 μl / 20 μl
Injection interval: 5 min / 8-, 5- or 3 min programe
UV/VIS-Detector: 415 nm / 415 nm
HPLC-Thermostat: 23 °C / 23 °C
Sample Preparation
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Diabetes,
Hemoglobin Testing
Hemoglobin
A1c ( HbA1c) in Whole Blood
(for VariantTM
and Modulary HPLC)
Reliable
and
cost-efficient analysis
“Gold standard”
for HbA1c analysis
Column lifetime: > 1000 analyses
This HbA1c test kit is
designed for the application on the BioRad analyzer VariantTM. However,
the kit can also be used with any conventional modular HPLC system.
Test Data
Linearity: The test is linear over the whole range of physiologically
occurring HbA1c values (approx. 3 - 16
% HbA1c).
Intraassay precision: 1 - 2 %
Interassay precision: 2 - 3 %
Dependence on the amount of total hemoglobin:
The HbA1c value is independent on the amount of total
hemoglobin in a range from 6 - 24 g/dl total hemoglobin.
HPLC Parameters
Pump: binary gradient pump, Flow rate: 2.0 ml/min
Injection volume: 20 μl
Injection interval: 3 min
UV/VIS-Detector: 415 nm
HPLC-Thermostat: 28 °C
Sample Preparation
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Metabolic
Diseases
5-Aminolevulinic
Acid /
Porphobilinogen in Urine
Easy to handle
Cost-efficient
Reliable test results
5-Aminolevulinic acid (5-ALA) and
porphobilinogen (PBG) are
intermediates in the biosynthesis of heme and
hemoglobin. In certain diseases, called porphyrias, an
accumulation of 5-ALA and PBG is observed
due to abnormalities in the heme production. Besides
to a genetic disposition (hereditary porphyrias),
porphyrias may also be induced by
several external factors, mostly by lead intoxication. Here,
an intoxication leads to an accumulation of
5-ALA by inhibition of the enzyme PBG synthase. In
contrast to the highly variable lead level in whole blood,
the urinary 5-ALA level remains increased for
at least one year after intoxication.
Test Data
Linearity: 0.8 - 80 mg/l
Recovery: > 90 %
Lower detection limit: 0.6 mg/l
Lower determination limit: 0.8 mg/l
Intraassay precision: 5-ALA: 3.4 %
PBG: 5.2 %
Interassay precision: 5-ALA: 4.6 %
PBG: 6.4 %
Sample Preparation
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Metabolic
Diseases
Homocysteine
in Plasma
High specific
derivatization
Run time: 5
min.
Sampling System for
pre-analytics available
Homocysteine is an established marker to estimate the
risk towards atherosclerosis and coronary heart disease.
An elevated plasma homocysteine level (hyperhomocysteinemia),
whether due to nutrient deficiencies
or by genetic defects, can be reduced by
intake of folic acid, vitamin B6 and B12. Therefore, the
determination of homocysteine does not only
serve for the risk assessment towards atherosclerotic affections
but also to ensure the proper combination
and dosage of a nutrition supplement therapy.
The present test kit allows the simultaneous
analysis of total homocysteine (tHcys) and
total cysteine (tCys). For preanalytics a special sampling
system is available.
Test Data
Linearity: 1 - 100 μmol/l
Recovery: 95 - 105 %
Lower detection limit: 0.5 μmol/l
Lower determination limit: 1 μmol/l
Intraassay precision: 3.9 %
Interassay precision: 5.8 %
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0
ml/min
Injection volume: 20 μl
Injection interval: 5 min
Fluorescence detector: 385 nm (exc.),
515 nm (em.)
HPLC-Thermostat: 30 °C
Sample Preparation
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Metabolic
Diseases
Hydroxyproline
in Urine
High analytical
selectivity
Run time: 8
min.
Reliable
determination
Osteoporosis is a metabolic bone
disease that is characterized by an abnormally low bone density and
therefore is associated with an increased risk of
fracture. The bone degradation goes along with an
increased formation of the amino acid hydroxyproline, which
is released into blood circulation and is
excreted with urine. The present test kit guarantees an
accurate hydroxyproline measurement. By a
special sample preparation, hydroxyproline is turned
into a derivative and can be quantified without interferences
from other amino acids.
Test Data
Linearity: 5 - 170 mg/l
Recovery: 90 - 100 %
Lower detection limit: 3 mg/l
Lower determination limit: 5 mg/l
Intraassay precision: 3.5 %
Interassay precision: 4.5 %
HPLC Parameters
Pump: binary gradient pump, flow
rates: 1.2, 1.4 ml/min
Injection volume: 20 μl
Injection interval: 8 min
UV/VIS-Detector: 471 nm
HPLC-Thermostat: 60 °C
Sample Preparation
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Metabolic
Diseases
Phenylalanine
(Phe, Tyr Trp) in Whole Blood, Plasma and from Filterspots
Easy sample
preparation
High analytical
sensitivity
Reliable monitoring
Several metabolic disorders (e.g.
phenylketonuria, tyrosinemia) result from an impaired amino acid metabolism.
Also, some forms of depression seem to
be connected with alterations of the metabolism of
tyrosine and tryptophane, being the precursors of
the brain neurotransmitters epinephrine, norepinephrine, dopamine
and serotonin. The present ClinRep®
complete kit serves for the monitoring of the
amino acids phenylalanine, tyrosine and tryptophane and
may be performed from whole blood, plasma
and filterspots (filter testcards).
Test Data
Linearity: 0.2 - 50 mg/dl
Recovery: 90 - 100 %
Lower detection limit: 0.1 mg/dl
Lower determination limit: 0.2 mg/dl
Intraassay precision:
Whole Blood: 5.2 %, Plasma: 2.9 %,
Filterspots: 3.8 %
Interassay precision:
Whole Blood: 6.8 %, Plasma: 4.5 %,
Filterspots: 5.4 %
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0 ml/min
Injection volume: 20 μl
Injection interval: 12 min
UV/VIS-Detector: 214 nm
HPLC-Thermostat: 30 °C
Sample Preparation
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Metabolic
Diseases
Prophyrins
Differentiated in Urine
High resolution of
the prophyrin profile
No interferences
High analytical
sensitivity
Metabolic diseases that lead to an
accumulation of porphyrins in blood and tissues are summarized in the
term “porphyrias”. The particular type
of porphyria is diagnosed by the
investigation of the specific porphyrin
excretion profile. For this purpose, the present
ClinRep® complete kit allows to separate (differentiate)
the diverse porphyrins with high resolution
(in different porphyrin-isomers!) and enables
an exact quantification. As quick tests for the
detection of porphyrias a column test for the determination
of the total porphyrins as well as a column test for the determination
of 5-aminolevulinic acid and porphobilinogen
are available.
Test Data
Linearity: 1 - 5000 μg/l
Lower detection limit: 0.5 μg/l
Lower determination limit: 1 μg/l
Intraassay precision: 2.5 %
Interassay precision: 2.9 %
HPLC Parameters
Pump: binary gradient pump,flow rate: 1.0 ml/min
Injection volume: 20 μl
Injection interval: 20 min
Fluorescence detector: 394 nm (exc.),
624 nm (em.)
Temperature: 25 °C (or ambient)
Sample Preparation
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Metabolic
Diseases
Total
Porphyrins in Urine
Quick performance
Easy handling
Low price per test
The determination of the urinary total porphyrins
serves for the basic diagnostics at
suspicion on porphyrias. For further
investigation in case of increased total
porphyrin levels, the urinary excretion profile should
be determined by HPLC. The present
test kit features in quick performance, easy
handling and a low price per analysis.
Test Data
Linearity: 20 - 4000 μg/l
Recovery: ~ 70 %
Lower detection limit: 15 μg/l
Lower determination limit: 20 μg/l
Intraassay precision: 5.5 %
Interassay precision: 7.2 %
Sample Preparation
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Tumor
Diseases (Biogenic
Amines)
Catecholamines
in Plasma
High sensitivity
Extraordinary low
detection limit
Specific sample
preparation
Epinephrine, norepinephrine and
dopamine are termed as catecholamines. The determination of catecholamines
is primarily used in order to help detect
and rule out pheochromocytomas in symptomatic patients,
i.e. in patients with persistent hypertension.
It is also used in order to help monitor for
recurrence when a pheochromocytoma has been discovered
and removed. For these purposes, the present
ClinRep® complete kit allows a quick and safe
quantification of the catecholamines. The kit is easy
to use and is characterized by an extraordinary good
lower detection limit.
Test Data
Linearity: 10 - 2500 ng/l
Recovery: 70 - 90 %
Lower detection limit: 5 ng/l
Lower determination limit: 10 ng/l
Intraassay precision:
6.7 % (norepinephr.), 7.6 %
(epinephrine), 6.1 % (dopamine)
Interassay precision:
5.3 % (norepinephr.), 4.2 %
(epinephrine), 3.9 % (dopamine)
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0
ml/min
Injection volume: 40 μl
Injection interval: 15 min
EC-Detector: potential: 500 mV,
Temperature: 25 °C (or ambient)
Sample Preparation
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Tumor
Diseases (Biogenic Amines)
Catecholamines
in Urine
Easy test handling
Reliable sample
preparation
High analytical
specificity
With this HPLC application, the
determination of the catecholamines is performed from urine. Whereas
the determination from plasma is most useful
for patients with persistent hypertension, the determination from urine
gives the amount that is excreted over a
24-hours period. Since the
catecholamine levels may fluctuate significantly
during this period, the determination from
urine may detect excess production that is missed
with the plasma testing. Here, the test kit ensures a chromatography
without interference due to an
optimized sample clean up.
Test Data
Linearity: 2 - 1000 μg/l
Recovery: 70 - 85 %
Lower detection limit: 1 μg/l
Lower determination limit: 2 μg/l
Intraassay precision:
3.8 % (norepinephr.), 5.6 %
(epinephrine), 5.8 % (dopamine)
Interassay precision:
5.6 % (norepinephr.), 4.9 %
(epinephrine), 4.7 % (dopamine)
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0
ml/min
Injection volume: 20 μl
Injection interval: 15 min
EC-Detector: potential: 500 mV
HPLC-Thermostat: 30 °C
Sample Preparation
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Tumor
Diseases (Biogenic Amines)
Catecholamines
in Urine-On-line Analysis with Fluorescence Detection
Fully automated
method
On-line sample
preparation
Robust analysis
The present ClinRep® complete kit is
a fully automated method for the catecholamine determination in
urine. A manual sample preparation is not required, but
is performed on-line within the HPLC system!
Test Data
Linearity: 2 - 1000 μg/l
Recovery: 100 %
Lower detection limit: 1 μg/l
Lower determination limit: 2 μg/l
Intraassay precision:
5.7 % (norepinephr.), 6.7 %
(epinephrine), 2.6 % (dopamine)
Interassay precision:
6.2 % (norepinephr.), 7.2 %
(epinephrine), 3.1 % (dopamine)
HPLC Parameters
Pump 1(SPE buffer): isocratic pump, flow
rate: 0.5 ml/min
Pump 2 (mobile phase): isocratic
pump, flow rate: 1.0 ml/min
Switching valves: 1 x switching valve 6-port / 3-channel
Injection volume: 100 μl
Injection interval: 28 min
Fluorescence detector: 275 nm (exc.),
330 nm (em.)
HPLC-Thermostat: 30 °C
Sample Preparation
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Tumor
Diseases (Biogenic Amines)
Metanephrines in Urine
Effective sample
clean-up
Sensitive
determination
Low cost per test
The term “metanephrines” is used
for the primary metabolites of the catecholamines, namely metanephrine,
normetanephrine, and 3-methoxytyramine.
The determination of catecholamines and
their metabolites serves for helping to
detect or rule out tumors of the
sympathoadrenal system (pheochromocytoma
and other neuroendocrine tumors). The
pattern and the periodicity of the secretion
of these substances into the blood circulation and
urine, respectively, depends on the particular tumor.
The reliable kit components of the present
test kit ensure a sensitive determination of the
analytes and enable a safe diagnosis.
Test Data
Linearity: 5 - 7500 μg/l
Recovery: 70 - 85 %
Lower detection limit: 3 μg/l
Lower determination limit: 5 μg/l
Intraassay precision: 2.4 % (normetanephr.),
4.1 % (metanephrine), 4.1 %
(3-methoxytyr.)
Interassay precision: 7.2 % (normetanephr.),
7.1 % (metanephrine), 5.3 %
(3-methoxytyr.)
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0
ml/min
Injection volume: 20 μl
Injection interval: 17 min
EC-Detector: potential: 720 mV
HPLC-Thermostat: 30 °C
Sample Preparation
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Tumor
Diseases (Biogenic Amines)
Serotonin
in Plasma
Quick sample
preparation
Run time: 10
min.
High analytical
sensitivity
Serotonin is a neurotransmitter in the central nervous system. Serotonin
deficiencies are connected with
disorders such as depression, schizophrenia, and
Parkinson's disease. Moreover, serotonin is produced
in excess by carcinoid tumors. Here, the determination
of serotonin, together with its metabolite 5-HIAA
is used for confirming or ruling out
this tumor. The present ClinRep® complete
kit allows a quick and highly sensitive determination
of the analyte. The sample preparation requires
just a single precipitation step. For the determination
from urine we offers an additional test
kit.
Test Data
Linearity: 2 - 1000 μg/l
Recovery: 80 - 90 %
Lower detection limit: 1 μg/l
Lower determination limit: 2 μg/l
Intraassay precision: 3 %
Interassay precision: 4 %
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0
ml/min
Injection volume: 20 μl
Injection interval: 10 min
EC-Detector: potential: 450 mV
HPLC-Thermostat: 30 °C
Sample Preparation
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Tumor
Diseases (Biogenic Amines)
Serotonin
in Urine
Easy sample
preparation
Highly selective
sample clean up.
Run time: 10
min.
Serotonin is an important neurotransmitter. The main metabolite of
serotonin is 5-hydroxyindole acetic
acid (5-HIAA) which is excreted with urine. Patients
with carcinoid tumors usually show increased
levels of serotonin in plasma and urine as
well as of 5-HIAA in urine. As some tumors show a decreased
serotonin turnover (e.g. AADC-deficient
tumors), low 5-HIAA levels must be
verified by the determination of serotonin.
The present test kit provides a
highly selective sample preparation and guarantees a
reliable chromatography.
Test Data
Linearity: 5 - 1000 μg/l
Recovery: 70 - 85 %
Lower detection limit: 3 μg/l
Lower determination limit: 5 μg/l
Intraassay precision: 3 %
Interassay precision: 4 %
HPLC Parameters
Pump: isocratic pump, flow
rate: 1.0 ml/min
Injection volume: 20 μl
Injection interval: 10 min
EC-Detector: potential: 450 mV
HPLC-Thermostat: 30 °C
Sample Preparation
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Tumor
Diseases (Biogenic Amines)
VMA,
HVA and 5-HIAA in Urine
Highly selective
sample preparation
Highly selective
sample clean up.
Run time: 10 min.
Vanillylmandelic acid (VMA) and
homovanillic acid (HVA) are end-products of the catecholamine
metabolism. The determination of VMA
and HVA in urine serves as screening
test for neuroblastoma, one of the
most common solid tumors of early childhood. 5-hydroxyindoleacetic
acid (5-HIAA) is a metabolite of
serotonin and is used for the diagnosis of carcinoid tumors.
The test procedure allows a reliable
determination of VMA, HVA and 5-HIAA
in only 15 min time for analysis.
Test Data
Linearity: 0.1 - 300 mg/l
Recovery: 70 - 90 %
Lower detection limit: 0.07 mg/l
Lower determination limit: 0.1 mg/l
Intraassay precision: 3 - 4 %
Interassay precision: 3 - 5 %
HPLC Parameters
Pump: isocratic pump, flow
rate: 0.9 ml/min
Injection volume: 20 μl
Injection interval: 15 min
EC-Detector: potential: 800 mV
HPLC-Thermostat: 30 °C
Sample Preparation
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Oxidative Stress
Malondialdehyde in Plasma/Serum
Robust chromatography
Injection interval:
5 min.
Malondialdehyde is
formed by radical mediated ClinChek® Plasma Control, Level II lipid
peroxidation (oxidative stress) and shows mutagenic and cytotoxics
effects by further reaction with DNA. For this reason, malondialdehyde
is considered as suitable biomarker to determine oxidative stress. The
ClinRep® complete kit allows the reliable quantification of
malondialdehyde from plasma and serum. For quality assurance a ClinCal®
plasma calibrator and ClinChek® plasma controls in two levels are
available.
Test Data
Linearity: 0.04 - 20 μmol/l
Recovery: 100 %
Lower detection limit: 0.02 μmol/l
Lower determination limit: 0.04 μmol/l
Intraassay precision: 4.5 %
Interassay precision: 8.7 %
HPLC Parameters
Pump: isocratic pump, flow rate: 0.8 ml/min
Injection volume: 20 μl
Injection interval: 5 min
Fluorescence detector: 515 nm (exc.), 553 nm (em.)
HPLC-Thermostat: 30 °C
Sample Preparation
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Vitamin-Status
Vitamin
A and E in Plasma
Quick sample
preparation
Run time: 8 min.
Reference materials
with excellent stability
Vitamin A (retinol) and vitamin E (a-tocopherol)
belong to the fat- soluble class of vitamins. In contrast to water-soluble
vitamins, an excessive administration may
lead to hypervitaminosis. Vitamin A deficiency is
the leading cause of preventable blindness in children
and raises the risk of disease and death from
severe infections. Vitamin E is an important antioxidant
and is thought to have a role in preventing atherosclerosis
by inhibiting oxidation of low-density
lipoprotein (LDL). The present ClinRep® complete
kit allows the simultaneous analysis of both
vitamins within a run time of 8 min. For sample preparation
just a single precipitation step is required.
Test Data
Linearity:
Vitamin A: 10 - 5000 μg/l,
Vitamin E: 0.4
- 150 mg/l
Recovery: 90 - 100 %
Lower detection limit: Vitamin A: 7 μg/l,
Vitamin E: 0.25 mg/l
Lower determination limit: Vitamin A: 10 μg/l, Vitamin
E: 0.4 mg/l
Intraassay precision: Vitamin A: 3.0 %, Vitamin
E: 2.4 %
Interassay precision: Vitamin A: 6.6 %,
Vitamin E: 4.4 %
HPLC Parameters
Pump: isocratic pump, flow rate: 1.5
ml/min
Injection volume: 20 μl
Injection interval: 8 min
UV-Detector: 325 / 295 nm
HPLC-Thermostat: 30 °C
Sample Preparation
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Vitamin-Status
Vitamin
B1 in Whole Blood
Determination as TPP
or total thiamine
Excellent
sensitivity: 0.5 / 0.2 mg/l
Run time: 4 min. / 6
min.
Combinable with B2
and B6 test kits
Vitamin B1 (thiamine, thiamine
pyrophosphate (TPP)) acts as
coenzyme in the carbohydrate metabolism and is essential for the normal
function of the organism. Deficiency
mainly occurs in undernourished people,
alcoholics or in special clinical situations (e.g.
hemodialysis). A fully developed deficiency syndrome
is called “BeriBeri”. With the present test kit,
vitamin B1 may be determined as TPP
or alternatively, by enzymatic treatment within sample
preparation, as total thiamine. The kit can be
easily combined with the test kits for vitamin B2 and
B6.
Test Data
Determination as TPP / as
Total Thiamine
Linearity: 1 - 200 μg/l / 0.5
- 160 μg/l
Recovery: 95 - 100 % / 95 - 100 %
Lower detection limit: 0.5 μg/l / 0.2
μg/l
Lower determination limit: 1 μg/l / 0.5
μg/l
Intraassay precision: 2.7 % / 2.8
%
Interassay precision: 4.2 % / 4.5
%
HPLC Parameters
Pump: isocratic pump, flow rate: 1.0
ml/min
Pump (Reagent N): flow rate: 0.1 ml/min
Injection volume: 50 μl
Injection interval: 4 min (TPP) / 6
min (total thiamine)
Fluorescence detector: 376 nm (exc.),
435 nm (em.)
HPLC-Thermostat: 35 °C
Sample Preparation
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Vitamin-Status
Vitamin
B2 in Whole Blood
Quick sample
preparation
Run time: 10 min.
Combinable with B1
and B6 test kits
The term “vitamin B2” covers three vitamers: riboflavin, flavin
mononucleotide (FMN) and flavin adenine
dinucleotide (FAD). FMN and FAD are the effective
forms of vitamin B2 and are synthesized in
vivo from riboflavin by phosphorylation. Vitamin B2
is important for energy production, enzyme function and
normal fatty acid and amino acid synthesis. The
undersupply with vitamin B2 is mostly attributed to
a general malnutrition. It is often seen in persons
with risk factors such as alcohol problems, advanced
age and extreme dietary habits. The present
test features in a short time for analysis (easy
sample preparation, short run time) and can be
easily combined with test kits for the vitamins B1
and B6.
Test Data
Determination as FAD
Linearity: 20 - 500 μg/l
Recovery: 95 - 100 %
Lower detection limit: 10 μg/l
Lower determination limit: 20 μg/l
Intraassay precision: 3.8 %
Interassay precision: 4.0 %
HPLC Parameters
Pump: isocratic pump, flow
rate: 1.0 ml/min
Injection volume: 50 μl
Injection interval: 10 min
Fluorescence detector: 450 nm (exc.),
530 nm (em.)
HPLC-Thermostat: 40 °C
Sample Preparation
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Vitamin-Status
Vitamin
B6 in Plasma/Whole Blood
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