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ClinMass® Applications
Immunosuppressants
Cyclosporine A, Tacrolimus, Sirolimus and Everolimus in Whole Blood –
On-Line Analysis
The ClinMass®
application for immunosuppressants represents a validated method for the
therapeutic drug monitoring (TDM) of cyclosporine A, tacrolimus,
sirolimus and everolimus. The sample preparation is based on a simple
pre-treatment step, followed by a fully automated on-line sample
analysis. The determination of the analytes is performed simultaneously
with an injection interval of just 5 min. For calibration and
measurement, we offer a 7 level whole blood calibrator set and two
internal standards. For quality control whole blood controls in three
different levels are available.
For cyclosporine A two additional levels (level IV, V) are offered.
Fully
automated on-line application
Two internal standards
7-Level
whole blood calibrator set
Whole
blood controls
in five levels
Test Data
Linearity:
up
to 4250 μg/l (Cyc), up to 200 μg/l (Tcr), up to 250 μg/l (Srl), up to
200 μg/l (Evr)
Lower detection limit: 5.7 μg/l (Cyc), 1.5 μg/l (Tcr), 0.74 μg/l (Srl),
1.2 μg/l (Evr)
Lower determination limit: 15.5 μg/l (Cyc), 4.0 μg/l (Tcr), 2.0 μg/l (Srl),
3.2 μg/l (Evr)
Intraassay precision: 3.7 % (Cyc), 2.8 % (Tcr), 3.3 % (Srl), 2.4 % (Evr)
Interassay precision: 5.1 % (Cyc), 4.3 % (Tcr), 3.9 % (Srl), 4.1 % (Evr)
LC-MS/MS Parameters
LC-Parameters
Pump 1: HPLC, flow rate 3 ml/min, water /
methanol (90:10)
Pump 2: HPLC, flow rate 0.5 ml/min, methanol / ammonium acetate buffer
(97:3)
Injection volume: 50 μl, Injection interval: 5 min
Switching valve: automatic switching valve, 6-port/3-channel
SPE column: Applied Biosystems Poros R1/20
Analytical column: Phenomenex Luna Phenyl-Hexyl
MS-Parameters
MS/MS-System: Applied Biosystems API 3000 Ion source: ESI positive
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Metabolic Diseases
Homocysteine in Plasma
Homocysteine is as an important risk factor for vascular diseases.
Elevated levels of homocysteine particularly have been shown to be
associated with an increased risk of coronary heart disease and a higher
likelihood of suffering a stroke. The ClinMass® application for
homocysteine represents a validated method for the quantification of
total plasma homocysteine by tandem mass spectrometry. After an easy and
fast two-step sample preparation (reduction, followed by precipitation
of the protein matrix), the samples are rapidly analysed by the LC-MS/MS
system within an injection interval of 2 min. For calibration and
measurement, we offer a plasma calibrator and an isotope labelled
internal standard. For quality control plasma controls in two different
levels are available.
Simple and quick two-step sample preparation
Injection interval: 2 min
Isotope labeled internal
standard, plasma
calibrator and plasma controls available
Test Data
Linearity: 0.6 - 92 μmol/l
Lower detection limit: 0.2 μmol/l, Lower determination limit: 0.6 μmol/l
Intraassay precision: 4.2 %, Interassay precision: 4.4 %
LC-MS/MS Parameters
LC-Parameters
Pump: HPLC, flow rate 0.7 ml/min, acetonitrile / water
Injection volume: 1 μl, Injection interval: 2 min
Analytical column: Supelcosil LC-CN
MS-Parameters
MS/MS-System: Applied Biosystems API 3000 Ion source: ESI positive
Sample Preparation
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