CSA | RAPID Optimization

‚ÄčProcedure for rapid method development within 1 hour

ChromSwordAuto | Rapid Optimization

The procedure is very valuable in early stages of method developments when it is necessary to obtain reasonable methods in a very short time. 

The 1st gradient run from low to high concentrations of organic modifier is aimed at understanding the sample and column properties.  The purpose of this run is to determine the eare and conditions when compounds of the sample are eluted.  The time needed to perform the experiment depends on the column length and flow rate.

CSA | RAPID Optimization

The 2nd run is a linear gradient and is based on the results of the 1st run. ChromSwordAuto makes decisions on the start and end concentration of the organic modifier and run time, depending on the resuts of the 1st run.

CSA | RAPID Optimization

After processing of the data from the 2nd run, ChromSword automatically creates retention models, simulates chromatograms under different conditions and makes decisions for the 3rd run, trying to separate all peaks with a target resolution.

CSA | RAPID Optimization

The main value of the rapid optimization is to reach a milestone for the analyst, before making future decisions:

  • change a combination of column / solvent / buffer if results are not acceptable
  • procees to FINE Optimization if results are acceptable but could be improved, or the sample should be checked for some unknown or not separated impurities
  • proceed to AutoRobust robustness testing if method is acceptable.