Venor® GeM-qEP:
Mycoplasma Detection Kit for qPCR

Type 1                          Type 2                          Pack Size
Cat.-No. 11-4025              Cat.-No. 11-5025              25 Tests
Cat.-No. 11-4100              Cat.-No. 11-5100              100 Tests
Cat.-No. 11-4250              Cat.-No. 11-5250              250 Tests

Description PCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Approved Validated according to the European Pharmacopoeia 2.6.7. for the testing of cell culture supernatants of master and production cells, chondrocytes for human application, sera, cell culture media, and therapeutic antibody formulations. Sensitive Individual primer probe sets maximize the sensitivity for Mycoplasma species, A. laidlawii and M. pneumoniae. Specific Validated for mycoplasma species listed in the European Pharmacopoeia: A. laidlawii, M. fermentans, M. hy orhinis, M. orale, M. pneumoniae, M. synoviae, M. argininii, Spiroplasma citri (add-on required). Detects more than 67 mycoplasma species. No detection of Arthrobacter, Burkholderia and other water born DNA contaminants. Simple Robust method, no need for general DNA extraction with cell culture supernatants. Reliable Kit contains internal control and positive control DNA and a specific internal control probe for optional PCR quality check.

 

Test Principle
Venor®GeM-qEP can be used for the detection of mycoplasma DNA in biological samples, like cell cultures. The specific primers amplify nucleotide sequences within a highly conserved region of the 16S rRNA gene specific for Mycoplasma species, M. pneumoniae and Acholeplasma laidlawii in individual PCR reactions which can be processed in the same cycler run. To rule out the presence of PCR inhibitors in the sample, an internal control is included in Venor®GeM-qEP. Venor®GeM-qEP can also be used with UNG for carry-over prevention. The optional Quantification Standards provide titrated DNA for the quantification of the mycoplasma DNA load in the sample.

 

Kit Components

• primers, probes and nucleotides at optimized concentration
• reaction buffer optimized for MB Taq DNA Polymerase
• positive control for easy result verification
• internal control to ensure accurate testing
• PCR Grade Water for resolving the components* and setting up the mastermixes
  

*Venor®GeM-qEP kit components are lyophilized and at +2 to +8 °C maintainable for at least 12 months.
Primer and nucleotides are aliquoted (á 25 tests) to guarantee consistent quality.
After re-hydratisation the reagents must be stored below -18 °C.

 

Instrument Compatibility

 

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