EUB Polymerase:
DNA free, use with Onar® EUB

Cat.-No. 54-0100              100 Units
Cat.-No. 54-0500              500 Units

DNA-free polymerase providing unmatched sensitivity

Description
EUB Polymerase is a thermostable DNA polymerase. It has a half-life of 3 hours at 96 °C.
EUB DNA Polymerase possesses the following activities: 5’ - 3’ DNA polymerase activity and 5’ - 3’ exonuclease activity. EUB Polymerase lacks the 3’ - 5’ proofreading activity, which makes it easy to use. EUB Polymerase is purified free of contaminating endo- and exonucleases. This enzyme is optimal for use with Onar®EUB.

Applications
Complex genomic or cDNA templates. Unit Definition One unit is defined as the amount of the enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72 °C.

Contents and Storage
EUB Polymerase, supplied at 100 units/ml, 2 units/μl in 20 mM Tris-HCl (pH 7.4 at 25 °C);
100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5 % Tween®20; 0.5 % Nonidet P 40; 200 μg/ml BSA, and
50 % (v/v) glycerol. Store below -18 °C.  

 

back to the to	back Contamination Control