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Contamination Control ...
   

Spread
Besides contamination with yeast, fungi and other cell types, the contamination of cell cultures with viruses, bacteria and mycoplasma is widely spread. Up to 87 % of all cell cultures in academic use and up to 4 % in industrial production are contaminat­ed with mycoplasma. Approx. 6 % of all cells used for therapeutic purposes are contami­nated with undetected bacteria. The regular use of antibiotics in cell culture media suppresses the bacterial growth to a level which are undetectable without a suitable detection method. 		Contamination Control Chart
Contamination control


Impact
It is important to keep an eye on these con­taminants during routine work out of diversified and severe reasons: Inhibition of cell proliferation up to 50 %, higher infection risk for patients treated with therapeutic cells, unspecific positive results in animal control testing by stimulated immune systems, influence of virus proliferation and infection rates, disturbance of the hybridoma-technique and patch-clamp experiments as well as a drastic decrease of electroporation rates. Mycoplasma can constitute up to 50 % of the total protein and 15 - 30 % of the DNA isolated from a cell culture. These impurities will complicate any downstream purification processes and cloning experi­ments.

Consequences
The PCR method can easily fulfill the restriction of the guideline in means of specificity and robustness. The sensitivity of the test method is limited by the low sample volume. This problem was solved by a cell culture enrichment which also solves another problem of direct testing: the detection of DNA from dead mycoplasma. Spiking a cell culture with 10 vital mycoplasma particles gives a strong detectable PCR signal within one week of cultivation; DNA from dead mycoplasma will be digested by extracellular nucleases.
A PCR method validated according to the guidelines of the Eur. Ph., e.g. Minerva Biolabs´ Venor®GeM-qEP for qPCR, is a powerful and easy to use tool for enhanced mycoplasma safety.

 

 

Contamination Control Product

Order No.

Tests

Venor® GeM
Mycoplasma Detection Kit for conventional PCR

11-1025
11-1050
11-1100
11-1250

25
50
100
250

Venor® GeM Advance
Convenient Mycoplasma Detection

11-7024
11-7048
11-7096
11-7240

24
48
96
240

Venor® GeM-qEP
Mycoplasma Detection Kit for qPCR

Type 1:
11-4025
11-4100
11-4250
Type 2:
11-5025
11-5100
11-5250

Type 1:
25
100
250
Type 2:
25
100
250

Mynox® Gold
Mycoplasma Elimination - 2 in 1 - Double Strength

10-0201
10-0501
10-1001

2 Applications
5 Applications
10 Applications

Mynox®
Mycoplasma Elimination - Free of Antibiotics

10-0200
10-0500
10-1000

2 Applications
5 Applications
10 Applications

Onar® EUB
Eubacteria Detection Kit for conventional PCR

12-1025
12-1050
12-1100
12-1250

25
50
100
250

Venor® MGS
Conventional PCR
for Mycoplasma gallisepticum & Mycoplasma synoviae

50-1050

50

Zell Shield
The Cell Culture Contamination Preventive

13-0050

50 ml

Mycoplasma-Off®
Disinfection Spray for the Removal of Mycoplasma

15-1000
15-5000

Spray bottle (1 L)
Refill bottles (5 x 1 L)

DNA Remover
Decontamination Spray for the Removal of DNA

 15-2025
15-2200		 Spray bottle (250 ml)
Refill bottles (4 x 500 ml)

EUB Polymerase
DNA free, use with Onar® EUB

 54-0100
54-0500		 100 Units
500 Units

PCR Ultra Pure Water

 55-0015
55-0075		 1.5 ml
5 x 1.5 ml

 

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