Onar® MRSA:
Methicillin resistant S. aureaus for qPCR

Cat.-No. 22-2025             25 Tests
Cat.-No. 22-2100             100 Tests
Cat.-No. 22-2250             250 Tests

Description
The Onar®MRSA is an in vitro test for quantitative and rapid PCR-based detection of Methicillin-resistant Staphylococcus aureus in clinical samples. This one-step triplex test system is a CE-marked IVD and approved for in vitro clinical diagnostics. The test system has undergone extensive validation programs.

Sensitive Detection of less than 50 MRSA particles per sample. Specific The Onar®MRSA is designed to amplify the Methicillin-resistant gene mecA. Staphylococcus aureus is confirmed by detecting the femA gene. Reliable and rapid The kit contains a positive control DNA and a specific internal control probe for optional PCR quality check. Results are obtained within 2-3 hrs. Convenient The convenient master mix format only requires a few pipetting steps.

 

Background
MRSA are specific strains of Staphy­lococcus aureus which have de­veloped antibiotic resistance to all penicillins, including methicillin and narrow-spectrum ß-lactamase-resistantpenicillin antibiotics. MRSA can infectother tissues when normal barriers have been breached (e.g. skin). This leads to furuncles, heavy infections and can lead to a sepsis. The Methicillin-resistant Staphylococcus aureus is de­scribed as a trigger for typical hospital related infections.

Kit Components

• primer, probes and nucleotides at
• optimized concentrations
• positive control for easy result
• verification
• internal control to prevent false
• negative results by PCR inhibition
• instruction manual
  
  Only purified DNA and Taq polymerase need to be added.
  Note: Onar®MRSA and Onar®Sa are validated and recommended for use with our reliable hot-start MB Taq DNA Polymerase (Cat.-No. 53-0200)

 

Test Principle
The Onar®MRSA is a test for semi-quantitative diagnostics of clinical samples. The test can basically be used with all relevant PCR machines. The supplied primer,probes and the positive control are specific for a segment of themecA gene of the Methicillin- resistant Staphylococcus aureus genome and the Staphylococcus aureus verifica­tion gene femA. The target probe emits fluorescent light at ~ 520 nm and the S. aureus confirmation emits fluores­cent light at ~ 560 nm. To rule out the presence of PCR inhibitors in the indi­vidual patient specimens, an internal control is included in the kit. The inter­nal control is detected by another probe emitting light at ~ 610 nm.

 

Instrument Compatibility

+ = recommended kit version
– = not compatible
o = untested but presumed to be compatible
V = validated

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