MB Taq DNA Polymerase
Unsupassable sensitivity for conventional and quantitative PCR

 

Cat.-No. 53-0050             50 units
Cat.-No. 53-0100             100 units
Cat.-No. 53-0200             200 units
Cat.-No. 53-0250             250 units

 

Description
Hot start MB Taq DNA Polymerase is the optimized mixture of Taq (Thermus aquaticus) DNA polymerase and anti-Taq DNA polymerase monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambi ent temperature (20-22 °C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70 °C. This enzyme is optimal for all Min erva Biolabs products except Onar®EUB. Complete activation of the polymerase is achieved at 94 °C for 2 min.

“Hot Start” Feature
The “hot start” Taq mixtures are designed to prevent unwanted amplification by preventing polymerase activity until the reaction mixture is above a certain temper ature (usually accomplished using a monoclonal antibody directed against Taq).

Application
Complex genomic or cDNA templates, low copy number targets, large numbers of thermal cycles, multiplex PCR.

Unit Definition
One unit is defined as the amount of the enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble DNA fraction in 30 minutes at 72 °C.

Contents and Storage
MB Taq DNA Polymerase, supplied at 5000 units/ml, 5 units/μl in 10 mM Tris-HCl (pH 7.0), 50 mM KCl,
0.1 mM EDTA, and 50 % (v/v) glycerol. 10x reaction buffer and 100 mM MgCl2 solution. Store below -18 °C.

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