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ClinMass© LC-MS/MS:
Ethylglucuronide and Ethylsulfate in Urine,
ClinMass© Complete Kit, Order No. MS8000

Ethylglucuronide and Ethylsulfate are known as relatively new, specific parameters for the assessment of alcohol consumption, which dates back several days. In Germany, they are particularly used for abstinence control in medical-psychological diagnostics of drivers aptitude.

With this ClinMass® Complete Kit RECIPE is offering the first commercially available CE/IVD test kit for LC-MS/MS systems.

For quantification Multi-Level Calibrators, isotope labelled Internal Standards and Urine Controls are available in the clinically relevant range. An Optimisation Mix is available for the MS/MS system.

The method validation of this ClinMass® Complete Kit complies with the requirements of the GTFCh directives for quality assurance in forensic-toxicological analyses.

 
  • First CE/IVD test kit for LC-MS/MS

  • Fast and easy sample preparation (dilution, injection)

  • Method validation according to GTFCh guidelines

Test Data

LC-MS/MS Parameters
Linearity:
-EtG: 76.2 - 10000 µg/l
-EtS: 21.8 - 76356 µg/l
LLOD:
-EtG: 45.7 µg/l
-EtS: 13.1 µg/l
LLOQ:
-EtG: 76.2 µg/l
-EtS: 21.8 µg/l
Intraassay precision:
EtG: 5.3 %
EtS: 4.6 %
Interassay precision:
EtG: 5.4 %
EtS: 4.9 %
LC-Parameters
HPLC pump: isocratic,
flow rates: 0.2 ml/min, 0.5 ml/min
Injection volume: 10 µl
Injection interval: 5 min
Column heater: 40 °C
MS-Parameters
Ion source: ESI negative
MS/MS-Mode: MRM

Sample Preparation

Ms8000_flowchart

 
Chromatogram of the ClinChek® Urine Control, Level II (order no. MS8081):
MS8000_chromatogram

 

ClinMass® Complete Kit for Ethylglucuronide and Ethylsulfate in Urine,
MS8000 Ordering Information

Order No. Description Quantity
MS8000 ClinMass® Complete Kit 1 pce.
for Ethylglucuronide and Ethylsulfate in Urine
for 400 assays
Contents (order no. MS8000):
MS8005 Autosampler Washing Solution 1000 ml
MS8010 Mobile Phase 1000 ml
MS8012 IS Internal Standard 100 ml within the kit: 4 x order no. MS8012
MS8713 Urine Calibrator Set, lyophil. 7 x 1 x 1 ml
Manual 1 pce.
Additional Components:
MS8014 Optimisation Mix 2 ml
MS8015 D Diluting Solution for Optimisation Mix 10 ml
MS8413 Urine Calibrator Set, lyophil. 4 x 1 x 1 ml
Start Accessories:
MS8030 Analytical Column with test chromatogram 1 pce.
ClinChek® Controls:
MS8080 Urine Control, lyophil., Level I 10 x 1 ml
MS8081 Urine Control, lyophil., Level II 10 x 1 ml
MS8082 Urine Control, lyophil., Level III 10 x 1 ml
MS8083 Urine Control, lyophil., Level I, II, III 3 x 3 x 1 ml




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