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CDT (Carbohydrate-Deficient Transferrin)
in Serum, Complete Kit, Order No. 21 000


CDT (carbohydrate deficient transferrin) is the most specific parameter for chronic alcohol abuse.

The test procedure of RECIPE closely directs to the recommendations of the IFCC-CDT-WG* for a "candidate reference method" and ensures a reliable separation of the transferrin-isoforms asialo- to hexasialotransferrin. The test kit can be applied for routine diagnostic as well as for confirmation analysis in case of unplausible diagnostic findings. The robust chromatography allows for a safe identification of genetic variants and thus prevents false diagnostic results. Besides to the diagnostic of alcohol abuse the test is suitable for the screening of CDG syndromes.

Alternatively, this application may also be performed as a fully automated method with on-line sample preparation („on-line“ analysis, order no. 21100).

*Working group of the IFCC for the international standardisation of CDT measurement."

  
  • Test procedure closely directed to the "candidate reference method" of the IFCC-CDT-WG*
    *Working group of the IFCC for the international standardisation of CDT measurement."

  • Quantification of all transferrin isoforms (incl. hexasialotransferrin)

  • Safe identification of genetic variants

  • Additionally available as fully automated method (on-line sample preparation)


Test Data

  

HPLC Parameters
Lower Detecrion limit: 0.1 %*
Lower determination limit: 0.3 %*
Intraassay precision: 4.2 %
Interassay precision: 4.3 %
*depends on the amount on total transferrin
 
Pump: Ternary gradient pump, flow
rates: 1.2, 2.0 ml/min
Injection volume: 400 - 500 µl
Injection interval: 20 min
UV/VIS-Detector: 460 nm (e.g. RECIPE Fixed-Wavelenght-VIS-Detector for CDT, FW3000)
HPLC Thermostat: 40 °C

 Sample Preparation

CDT_Serum

 

Chromatograms
Native sample, normal range Native sample, pathological range
 
Disialotransferrin: 6.22 min, Trisialotransferrin: 6.85min, Terasialotransferrin: 7.73 min, Pentasialotransferrin: 8.75 min, Hexasialotransferrin: 9.65 min   Asialotransferrin: 4.25 min, Disialotransferrin: 5.98 min, Trisialotransferrin: 6.71 min, Terasialotransferrin: 7.69 min, Pentasialotransferrin: 8.66 min, Hexasialotransferrin: 9.57 min