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CDT (Carbohydrate-Deficient Transferrin) in Serum - Automated On-Line Analysis, Complete Kit,
Order No. 21 200


This test kit is designed as a fast on-line method for CDT analysis (injection interval: 9.5 min). Contrary to the conventional method (order no. 21000), a manual sample preparation is not required. The sample preparation is carried out through the HPLC system by column switching using a special pre-column, where the serum matrix is separated from the analytes. Prior to injection the samples simply have to be diluted and incubated (see below "sample pretreatment").

Like the CDT test kit with order no. 21000, this analytical method widely complies with the candidate reference method of the IFCC-CDT-WG*.

*Working group of the IFCC for the international standardisation of CDT measurement.

 
  • Sample preparation via column switching

  • Injection interval: 9.5 min

  • Widely complies with the "candidate reference method" of the IFCC-CDT-WG*


Test Data

HPLC Parameters
Lower detection limit: 0.1 %*
Lower determination limit: 0.3 %*
Intraassay precision: 4.5 %
Interassay precision: 3.9 %
*depending on the amount of total transferrin and on detector sensitivity
Pump 1,
Mobile Phases A and B:

binary gradient pump,
flow rate: 1.4 ml/min
Pump 2,
Mobile Phase C :

isocratic pump,
flow rate: 0.4 ml/min
Injection volume: 400 - 500 µl
Injection interval: 12 or 9.5 min*
*The chromatograpic separation can be performed with a 12- or 9.5 min program
UV/VIS-Detector: 460 nm
HPLC-Thermostat: 40 °C

 

 Sample Pre-treatment


 

Chromatograms (9.5 min. program)
Native sample, normal range Native sample, pathological range
 
Disialotransferrin: 4.50 min, Trisialotransferrin: 5.17 min, Terasialotransferrin: 6.17 min, Pentasialotransferrin: 7.13 min, Hexasialotransferrin: 7.86 min   Disialotransferrin: 4.50 min, Trisialotransferrin: 5.17 min, Terasialotransferrin: 6.17 min, Pentasialotransferrin: 7.13 min, Hexasialotransferrin: 7.86 min