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Catecholamines in Plasma
Complete Kit, Order No. 1000

 

Epinephrine, norepinephrine and dopamine are termed as catecholamines. The determination of catecholamines is primarily used for helping to detect and rule out pheochromocytomas in symptomatic patients, i.e. in patients with persistent hypertension. It is also used in order to help monitor for recurrence when a pheochromocytoma has been discovered and removed.

For these purposes the present ClinRep® complete kit allows a quick and safe quantification of the catecholamines. The kit is easy to use and is characterized by an extraordinary good lower detection limit.

  
  • Reliable sample preparation

  • Quick and safe quantification


  • Detection limit: 5 ng/l


Test Data

  

HPLC Parameters
Linearity: 10 - 2500 ng/l
  (per analyte)
Recovery: 70 - 90 %
Lower detection limit: 5 ng/l
  (per analyte)
Lower determination limit 10 ng/l
  (per analyte)
Intraassay precision: 6.7 % (norepinephr.)
  7.6 % (epinephrine)
  6.1 % (dopamine)
Interassay precision: 5.3 % (norepinephr.)
  4.2 % (epinephrine)
  3.9 % (dopamine)
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 40 µl (sample)
10 µl (standard solution, order no. 1011)
Injection interval: 15 min
EC-Detector: potential: 500 mV,
sensitivity: 10 nA (settings
for RECIPE detector EC3000
Column heater: 25°C (or ambient temperature)

Sample Preparation

1000_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
1000_chrom1   1000_chrom2
Norepinephrine: 4.28 min, Epinephrine: 5.08 min, IS: 7.55 min, Dopamine: 12.05 min   Norepinephrine: 4.28 min, Epinephrine: 5.07 min, IS: 7.56 min, Dopamine: 12.02 min

 

Catecholamines in Plasma
Complete Kit, Order No. 1000

Ordering Information

Order No. Description Quantity
1000  ClinRep® Complete Kit for Catecholamines in Plasma for
250 assays for 250 assays		 1 pce. 
 
  Kit Components (order no. 1000):  
1210 Mobile Phase 1000 ml
1011 Standard Solution 10 ml
1012 IS Internal Standard 15 ml
1013 Plasma Calibrator, lyophil. 5 x 5 ml
1020 Sample Preparation Columns incl. elution vials 50 pcs.
within the kit: 5 x order no. 1020
1021 W Washing Solution 250 ml
within the kit: 3 x order no. 1021
1022 E Eluting Reagent 30 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
1030 Analytical Column with test chromatogram 1 pce.
 
  Accessories:  
1041 2 ml sample vial with cap for storing samples 50 pcs.
1042 Sampling system for Plasma Catecholamines (5 ml) 50 pcs.
1060 Sample preparation column, empty with frit and cap 50 pcs.
1061 250 µl elution vial with cap 50 pcs.
 
  ClinChek® Controls:  
8810 Plasma Control, lyophil., Level I 10 x 5 ml
8811 Plasma Control, lyophil., Level II 10 x 5 ml
8809 Plasma Control, lyophil., Level I, II 2 x 5 x 5 ml