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VMA, HVA and 5-HIAA in Urine
Complete Kit, Order No. 3000

 

Vanillylmandelic acid (VMA) and homovanillic acid (HVA) are end-products of the catecholamine metabolism. The determination of VMA and HVA in urine serves as screening test for neuroblastoma, one of the most common solid tumors of early childhood. 5-hydroxyindoleacetic acid (5-HIAA) is a metobolite of serotonin and is used for the diagnosis of carcinoid tumors (see order nos.: 6000 and 7000).

Here, the test kit of RECIPE enables a safe determination of urinary VMA, HVA and 5-HIAA. Alternatively to the present method with manual sample preparation, RECIPE offers test kits for the use with automatic pipetting systems from RECIPE (order nos. 3300-3400) and Gilson® (order nos. 3100-3200).

  
  • Robust test procedure

  • Highly selective sample preparation

  • Injection interval: 15 min

  • Test kits for automatic pipetting systems of Gilson and RECIPE available


Test Data

  

HPLC Parameters
Linearity: VMA: 0.6 - 100 mg/l
  HVA: 0.3 - 100 mg/l
  5-HIAA: 0.2 - 50 mg/l
Recovery: 75 - 90 %
Lower detection limit: VMA: 0.2 mg/l
  HVA: 0.1 mg/l
  5-HIAA: 0.1 mg/l
Lower determination limit: VMA: 0.6 mg/l
  HVA: 0.3 mg/l
  5-HIAA: 0.2 mg/l
Intraassay precision: VMA: 2.6 %
  HVA: 2.5 %
  5-HIAA: 3.0 %
Interassay precision: VMA: 3.7 %
  HVA: 3.8 %
  5-HIAA: 3.6 %
 
Pump: isocratic pump,
flow rate: 0.9 ml/min
Injection volume: 20 µl
Injection interval: 15 min
EC-Detector: potential: 800 mV,
sensitivity: 50 nA (settings
for RECIPE detector EC3000)
HPLC-Thermostat: 30°C

 

Sample Preparation

3000_flow

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
3000_chrom1   3000_chrom2
VMA: 3.67 min, IS: 5.50 min, HVA: 7.99 min,
5-HIAA: 11.80 min
   VMA: 3.62 min, IS: 5.46 min, HVA: 7.96 min,
5-HIAA: 11.77 min

 

VMA, HVA and 5-HIAA in Urine
Complete Kit, Order No. 3000

Ordering Information

Order No. Description Quantity
3000  ClinRep® Complete Kit for VMA, HVA and 5-HIAA in Urine for 100 assays 1 pce. 
 
  Kit Components (order no. 3000):  
3010 Mobile Phase 1000 ml
3011 Standard Solution, lyophil. 5 x 1 ml
3012 IS Internal Standard 100 ml
3013 Urine Calibrator, lyophil. 5 x 5 ml
3020 Sample Preparation Columns 50 pcs.( within the kit: 2 x order no. 3020)
3021 A Ammonia Solution 100 ml
3022 B Boric Acid Solution 300 ml (within the kit: 2 x order no. 3022)
3023 E Eluting Reagent 100 ml ( within the kit: 2 x order no. 3023)
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
3030 Analytical Column with test chromatogram 1 pce.
 
  ClinChek® Controls:  
8820 Urine Control, lyophil., Level I 10 x 8 ml
8821 Urine Control, lyophil., Level II 10 x 8 ml
8822 Urine Control, lyophil., Level I, II 2 x 5 x 8 ml