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VMA, HVA and 5-HIAA in Urine | Automated Sample Preparation with ClinLab® APS3000
Complete Kit, Order No. 3300 and 3400

 

As an alternative to the determination of VMA, HVA and 5-HIAA with manual sample preparation (see ClinRep® complete kit with order no. 3000), RECIPE offers appropriate ClinRep® complete kits for the use with automatic pipetting systems from Gilson® (see order nos. 3100-3200) and RECIPE (see below). There, the sample preparation and -injection is performed fully automated.

The ClinRep® complete kit for the determination of VMA, HVA and 5-HIAA with the automatic pipetting system RECIPE ClinLab® APS3000 contains all reagents, that are required for analysis, including instrument adapted sample preparation columns.

 
  • Automated sample preparation

  • Robust chromatography

  • Testkits available for 100 and 500 assays


Test Data

  

HPLC Parameters
Linearity: VMA: 0.9 - 50 mg/l
  HVA: 0.9 - 50 mg/l
  5-HIAA: 0.5 - 50 mg/l
Recovery: 75 - 90 %
Lower detection limit: VMA: 0.3 mg/l
  HVA: 0.3 mg/l
  5-HIAA: 0.2 mg/l
Lower determination limit: VMA: 0.9 mg/l
  HVA: 0.9 mg/l
  5-HIAA: 0.5 mg/l
Intraassay precision: VMS: 2.6 %
  HVA: 3.2 %
  5-HIAA: 2.8 %
Interassay precision: VMA: 2.2 %
  HVA: 2.6 %
  5-HIAA: 2.3 %
 
Pump: isocratic pump,
flow rate: 0.9 ml/min
Injection volume: 20 µl
Injection interval: 16 min
EC-Detector: potential: 800 mV,
sensitivity: 100 nA
(settings
for RECIPE detector EC3000)
HPLC-Thermostat:: 30°C

 

Sample Preparation

 

sample preparation

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
 
VMA: 3.67 min, IS: 5.50 min, HVA: 7.99 min,
5-HIAA: 11.80 min
   VMA: 3.62 min, IS: 5.46 min, HVA: 7.96 min,
5-HIAA: 11.77 min


VMA, HVA and 5-HIAA in Urine | Automated Sample Preparation with ClinLab® APS3000
Complete Kit, Order No. 3300 and 3400

Ordering Information

Order No. Description Quantity
3300   ClinRep® Complete Kit for VMA, HVA and 5-HIAA in Urine(automated sample preparation with ClinLab® APS3000) for 100 assays 1 pce.  
 
  Kit Components (order no. 3300):  
3010 Mobile Phase 1000 ml
3011 Standard Solution, lyophil. 5 x 1 ml
3012 IS Internal Standard 100 ml
3013 Urine Calibrator, lyophil. 5 x 5 ml
3320 Sample Preparation Columns 50 pcs. (within the kit: 2 x order no. 3320)
3021 A Ammonia Solution 100 ml
3022 B Boric Acid Solution 300 ml (within the kit: 2 x order no. 3022)
3023 E Eluting Reagent 100 ml (within the kit: 2 x order no. 3023)
FK6401 Sample vial, clear glass, 4 ml 100 pcs.
(within the kit: 2 x order no. FK6401)
FK6402 Sample vial without cap, PP, 1.5 ml 100 pcs.
  Manual 1 pce.
  Quick Reference 1 pce.
 
3400   ClinRep® Complete Kit for VMA, HVA and 5-HIAA in Urine(automated sample preparation with ClinLab® APS3000) for 500 assays 1 pce.  
     
  Kit Components (order no. 3400):  
3010 Mobile Phase 1000 ml (within the kit: 5 x order no. 3010)
3011 Standard Solution, lyophil. 5 x 1 ml (within the kit: 5 x order no. 3011)
3012 IS Internal Standard 100 ml ( within the kit: 5 x order no. 3012)
3013 Urine Calibrator, lyophil. 5 x 5 ml (within the kit: 5 x order no. 3013)
3320 Sample Preparation Columns 50 pcs. (within the kit: 10 x order no. 3320)
3021 A Ammonia Solution 100 ml (within the kit: 5 x order no. 3120)
3022 B Boric Acid Solution 300 ml (within the kit: 10 x order no. 3022)
3023 E Eluting Reagent 100 ml (within the kit: 10 x order no. 3023)
FK6401 Sample vial, clear glass, 4 ml 100 pcs.
(within the kit: 10 x order no. FK6401)
FK6402 Sample vial without cap, PP, 1.5 ml 100 pcs.
(within the kit: 5 x order no. FK6402)
  Manual 1 pce.
  Quick Reference 1 pce.
     
  Start Accessories:  
3030 Analytical Column with test chromatogram 1 pce.
 
  Refill Service:  
3031 Analytical Column with test chromatogram 1 pce.
  (only if you send back your original column)  
     
  ClinChek® Controls:  
8820 Urine Control, lyophil., Level I 10 x 8 ml
8821 Urine Control, lyophil., Level II 10 x 8 ml
8822 Urine Control, lyophil., Level I, II 2 x 5 x 8 ml