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Serotonin in Plasma
Complete Kit, Order No. 6000

 

Serotonin is a neurotransmitter in the central nervous system. Serotonin deficiencies are connected with disorders such as depression, schizophrenia, and Parkinsons's disease. Moreover, serotonin is produced in excess by carcinoid tumors. Here, the determination of serotonin, together with its metabolite 5-HIAA (see order no. 3000), is used for confirming or ruling out this tumor.

The present ClinRep® complete kit allows a quick and highly sensitive determination from platelet-rich or platelet-poor plasma and serum. The sample preparation requires just a single precipitation step. For the determination from urine RECIPE offers an additional test kit. (see order no. 7000).

  
  • Quick and easy sample preparation
    (protein precipitation)

  • Robust chromatography

  • High analytical sensitivity

  • Injection interval: 10 min


Test Data

  

HPLC Parameters
Linearity: 1 - 1000 µg/l
Recovery: 80 - 90 %
Lower detection limit: 0.5 µg/l
Lower determination limit: 1 µg/l
Intraassay precision: 3 %
Interassay precision: 4 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 10 min
EC-Detector: potential: 450 mV,
sensitivity: 20 nA (settings
for RECIPE detector EC3000
HPLC-Thermostat: 30°C

 

Sample Preparation

6000_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
6000_chrom1   6000-chrom2
Serotonin: 5.56 min, IS: 7.13 min   Serotonin: 5.56 min, IS: 7.13 min


Serotonin in Plasma
Complete Kit, Order No. 6000

Ordering Information

Order No. Description Quantity
6000  ClinRep® Complete Kit for Serotonin in Plasmafor 100 assays 1 pce. 
 
  Kit Components (order no. 6000):  
6710 Mobile Phase 1000 ml
6711 Standard Solution, lyophil. 5 x 2 ml
6712 IS Internal Standard, lyophil. 5 x 1 ml
6013 Plasma Calibrator, lyophil. 5 ml
6020 Sample Preparation Vials 100 pcs.
6021 P Precipitant 22 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
6030 Analytical Column with test chromatogram 1 pce.
 
  ClinChek® Controls:  
8810 Plasma Control, lyophil., Level I 10 x 5 ml
8811 Plasma Control, lyophil., Level II 10 x 5 ml
8809 Plasma Control, lyophil., Level I, II 2 x 5 x 5 ml