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Serotonin in Urine
Complete Kit, Order No. 7000

Serotonin is an important neurotransmitter. The main metabolite of serotonin is 5-hydroxyindole acetic acid (5-HIAA) which is excreted with urine. Patients with carcinoid tumors usually show increased levels of serotonin in plasma and urine as well as of 5-HIAA in urine (see order no. 3000). As some tumors show a decreased serotonin turnover (e.g. AADC-deficient tumors), low 5-HIAA levels should be verified by the determination of serotonin.

For this purpose, the test kit of RECIPE provides a highly selective sample preparation and thus guarantees a reliable chromatography.

 
  • Reliable sample preparation


  • Robust chromatography


  • Injection interval: 10 min.


Test Data

  

HPLC Parameters
Linearity: 5 - 1000 µg/l
Recovery: 70 - 85 %
Lower detection limit: 3 µg/l
Lower determination limit: 5 µg/l
Intraassay precision: 3 %
Interassay precision: 4 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 10 min
EC-Detector: potential: 450 mV,
sensitivity: 50 nA (settings
for RECIPE detector EC3000)
HPLC-Thermostat: 30°C

 

Sample Preparation

7000_flow

 

Chromatograms
ClinChek® Urine Control, Level I ClinCheck® Urine Control, Level II
7000_chrom1   7000_chrom2
Serotonin: 4.13 min, Internal Standard IS: 5.31 min   Serotonin: 4.11 min, Internal Standard IS: 5.31 min