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Phenylalanine, Tyrosine and Tryptophane
in Whole Blood, Plasma and from Filterspots
Complete Kit, Order No. 10 000

 

Several metabolic disorders result from an impaired amino acid metabolism. Also, some forms of depression seem to be connected with alterations of the metabolism of tyrosine and tryptophane, being the precursors of the brain neurotransmitters epinephrine, norepinephrine, dopamine and serotonin.

The ClinRep® complete kit of RECIPE serves for the monitoring of the amino acids phenylalanine, tyrosine and tryptophane and may be performed from whole blood, plasma and filterspots (filter testcards).

  
  • Easy sample preparation

  • High analytical sensitivity


Test Data

  

HPLC Parameters
Linearity: 0.2 - 50 mg/dl
Recovery: 90 - 100 %
Lower detection limit: 0.1 mg/dl
Lower determination limit: 0.2 mg/dl
Intraassay precision: Whole Blood: 5.2 %
  Plasma: 2.9 %
  Filterspots: 3.8 %
Interassay precision: Whole Blood: 6.8 %
  Plasma: 4.5 %
  Filterspots: 5.4 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 12 min
UV/VIS detector: 214 nm
HPLC-Thermostat: 30°C

 

Sample Preparation

10000_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
10000_chrom1   10000_chrom2
Tyrosine: 2.18 min, Phenylalanine: 3.84 min,
IS 7.11 min, Tryptophane: 9.76 min		   Tyrosine: 2.18 min, Phenylalanine: 3.85 min,
IS 7.14 min, Tryptophane: 9.80 min


Phenylalanine, Tyrosine and Tryptophane
in Whole Blood, Plasma and from Filterspots
Complete Kit, Order No. 10 000

Ordering Information

Order No. Description Quantity
10000  ClinRep® Complete Kit for Phe, Tyr and Trp for 100 assays 1 pce. 
 
  Kit Components (order no. 10000):  
10010 Mobile Phase 1000 ml
10011 Standard Solution 4 ml
10013 Plasma Calibrator, lyophil. 3 ml
10020 Sample Vials with Precipitation Reagent and Internal Standard IS 50 pcs.
within the kit: 2 x order no. 10020
10031 Filter Testcards 100 pcs.
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
10030 Analytical Column with test chromatogram 1 pce.
FK7000 manu-Cart® cartridgeholder, on time accessory 1 set
 
  Accessories:  
FK3000 Thermocult Incubator (37° C) 1 pce.
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
10080 Plasma Control, lyophil., Level I 10 x 8 ml
10081 Plasma Control, lyophil., Level II 10 x 8 ml
10082 Plasma Control, lyophil., Level I, II 2 x 5 x 8 ml
10182 Whole Blood Control (filter spot), Level I, II 2 x 3 x 3 spots


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