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Coenzyme Q10 in Whole Blood / Plasma / Serum
Complete Kit, Order No. 31 000

 

Coenzyme Q10 (CoQ10) plays an outstanding role in the energy production within the cell metabolism. In addition, CoQ10 is an important antioxidant and thus protects the cell from radical oxidation (oxidative stress). Lowered blood concentrations of CoQ10 seem to be connected with the pathogenesis of a large number of diseases. On the other hand, CoQ10 deficiencies are known to be the result of a pathological condition, such as in cardiomyophathies. There, a deficient CoQ10 status may be impaired by therapy with certain lipid-lowering agents (statins) and thus requires monitoring of the CoQ10 level. For this purpose, the RECIPE test kit allows for a reliable determination of CoQ10 in whole blood, plasma and serum. For quality assurance of the analytical results, reference materials (Test Solution, Plasma Calibrator and Plasma Controls) with excellent stability are available.

  
  • Reliable sample preparation

  • Robust chromatography

  • Reference materials with excellent stability


Test Data

  

HPLC Parameters
Linearity: 38.9 - 20000 µg/l
Recovery: 94 - 108 %
Lower detection limit: 19.5 µg/l
Lower determination limit: 38.9 µg/l
Intraassay precision: 3.4 %
Interassay precision: 5.0 %
 
Pump: isocratic pump,
flow rate: 1.4 ml/min
Injection volume: 30 µl
Injection interval: 14 min
UV - Detector: 275 nm
HPLC-Thermostat: 30°C

 

Sample Preparation

31000_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
31000_chrom1   31000_chrom2
IS: 7.34 min, CoQ10: 11.54 min   IS: 7.32 min, CoQ10: 11.49 min


Coenzyme Q10 in Whole Blood / Plasma / Serum
Complete Kit, Order No. 31 000

Ordering Information

Order No. Description Quantity
31000  ClinRep® Complete Kit for Coenzyme Q10 in Whole Blood / Plasma / Serum for 100 assays 1 pce..
 
  Kit Components (order no. 31000):  
31010 Mobile Phase 1000 ml
31012 IS Internal Standard 40 ml
31013 Plasma Calibrator, lyophil. 5 x 2 ml
31014 Test Solution 3 ml
31020 Sample Preparation Vials 100 pcs.
31021 P Precipitant 50 ml
31022 O Oxidation Reagent 5 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
31030 Analytical Column with test chromatogram 1 pce.
31032 Guard Column Holder incl. 1 Guard Column 1 pce.
 
  Accessories:  
31033 Guard Column 5 pcs.
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
31080 Plasma Control, lyophil., Level I 10 x 2 ml
31081 Plasma Control, lyophil., Level II 10 x 2 ml
31082 Plasma Control, lyophil., Level I, II 2 x 5 x 2 ml

 

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