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Malondialdehyde in Plasma / Serum
Complete Kit, Order No. 32 000

 

Malondialdehyde is formed by radical mediated lipid peroxidation (oxidative stress) and shows mutagenic and cytotoxics effects by further reaction with DNA. For this reason, malondialdehyde is considered as suitable biomarker to determine oxidative stress.

The ClinRep® complete kit of RECIPE allows the reliable quantification of malondialdehyde from plasma and serum. For quality assurance a ClinCal® plasma calibrator and ClinChek® plasma controls in two levels are available.

  
  • Reliable sample preparation

  • Robust chromatography

  • Injection interval: 5 min


Test Data

  

HPLC Parameters
Linearity: 0.04 - 20 µmol/l
Recovery: 100 %
Lower detection limit: 0.02 µmol/l
Lower determination limit: 0.04 µmol/l
Intraassay precision: 3.8 %
Interassay precision: 7.4 %
 
Pump: isocratic pump,
flow rate: 0.8 ml/min
Injection volume: 20 µl
Injection interval: 5 min
Fluorescence detector: 515 nm (exc.), 553 nm (em.)
HPLC-Thermostat: 30°C

 

Sample Preparation

32000_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
32000_chrom1   32000_chrom2
Malondialdehyde: 2.75 min   Malondialdehyde: 2.75 min


Malondialdehyde in Plasma / Serum
Complete Kit, Order No. 32 000

Ordering Information

Order No. Description Quantity
32000  ClinRep® Complete Kit f. Malondialdehyde in Plasma / Serum for 100 assays 1 pce..
 
  Kit Components (order no. 32000):  
32010 Mobile Phase 1000 ml
32013 Plasma Calibrator, lyophil. 5 x 0.5 ml
32020 Derivatisation Vials 100 pcs.
32021 Sample Preparation Vials 100 pcs.
32022 Reagent A 70 ml
32023 Reagent B 20 ml
32024 Reagent C 20 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
32030 Analytical Column with test chromatogram 1 pce.
32031 Guard Column 5 pcs.
FK7000 manu-Cart® cartridgeholder, one time accessory 1 set
 
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
32080 Plasma Control, lyophil., Level I 10 x 0.5 ml
32081 Plasma Control, lyophil., Level II 10 x 0.5 ml
32082 Plasma Control, lyophil., Level I, II 2 x 5 x 0.5 ml


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