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Vitamins A and E in Plasma
Complete Kit, Order No. 22 000

 

Vitamin A (retinol) and vitamin E (α-tocopherol) belong to the fatsoluble class of vitamins. In contrast to watersoluble vitamins, an excessive administration may lead to hypervitaminosis. Vitamin A deficiency is the leading cause of preventable blindness in children and raises the risk of disease and death from severe infections. Vitamin E is an important andioxidant and is thought to have a role in preventing atherosclerosis by inhibiting oxidation of low-density lipoprotein (LDL).

The ClinRep® complete kit of RECIPE allows the simultaneous analysis of both vitamins within a run time of 8 min. For quality assurance reference materials with excellent stability are available.

  
  • Quick and easy sample preparation
    (protein precipitation)


  • Fast chromatography
    (injection interval: 8 min)


  • Reference materials with excellent stability available


Test Data

  

HPLC Parameters
Linearity: Vitamin A: 0.02 - 5.0 mg/l
  Vitamin E: 0.40 - 50 mg/l
Recovery: 92 - 98 %
Lower detection limit: Vitamin A: 0.01 mg/l
  Vitamin E: 0.30 mg/l
Lower determination limit: Vitamin A: 0.02 mg/l
  Vitamin E: 0.40 mg/l
Intraassay precision: Vitamin A: 1.4 %
  Vitamin E: 1.4 %
Interassay precision: Vitamin A: 5.0 %
  Vitamin E: 3.2 %
 
Pump: isocratic pump,
flow rate: 1.5 ml/min
Injection volume: 50 µl
Injection interval: 8 min
UV detector: 325 / 295 nm
HPLC-Thermostat: 30°C

 

Sample Preparation

22000_flow

 

Chromatograms
ClinChek® Serum Control, Level I ClinCheck® Serum Control, Level II
22000_chrom1   22000_chrom2
Vitamin A: 1.82 min, IS: 4.91 min, Vitamin E: 7.20 min   Vitamin A: 1.82 min, IS: 4.91 min, Vitamin E: 7.19 min


Vitamins A and E in Plasma
Complete Kit, Order No. 22 000

Ordering Information

Order No. Description Quantity
22000  ClinRep® Complete Kit for Vitamins A and E in Plasma for 100 assays 1 pce. 
 
  Kit Components (order no. 22000):  
22010 Mobile Phase 1000 ml
22011 Standard Solution 3 ml
22012 P Precipitant with Internal Standard IS 15 ml
22013 Serum Calibrator, lyophil. 3 ml
22020 Sample Preparation Vials with Reagent, lyophil. 50 pcs.
within the kit: 2 x order no. 22020
22021 Reaction Vials 100 pcs.
22022 S Stabilizing Reagent 10 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
22030 Analytical Column with test chromatogram 1 pce.
FK7000 manu-CART® cartridgeholder, one time accessory 1 set
 
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
8870 Serum Control, lyophil., Level I 10 x 5 ml
8871 Serum Control, lyophil., Level II 10 x 5 ml
8872 Serum Control, lyophil., Level III 10 x 5 ml
8873 Serum Control, lyophil., Level I, II, III 3 x 3 x 5 ml


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