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Vitamin B2 in Whole Blood
Complete Kit, Order No. 25 000

 

The term "vitamin B2" covers three vitamers: riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). FMN and FAD are the effective forms of vitamin B2 and are synthesized in vivo from riboflavin by phosphorylation. Vitamin B2 is important for energy production, enzyme function and normal fatty acid and amino acid synthesis. The undersupply with vitamin B2 is mostly attributed to a general malnutrition. It is often seen in persons with risk factors such as alcohol problems, advanced age und extreme dietary habits.

The test kit of RECIPE features in a short time for analysis (easy sample preparation, short run time) and can be easily combined with test kits for the vitamins B1 and B6 (see combi kit, order no. 27000).

  
  • Quick and easy sample preparation


  • Fast chromatography
    (injection interval: 10 min)


  • Combineable with the test kits for vitamins B1 and B6


Test Data
Determination as FAD

  

HPLC Parameters
Linearity: 20 - 500 µg/l
Recovery: 95 - 100 %
Lower detection limit: 10 µg/l
Lower determination limit: 20 µg/l
Intraassay precision: 3.8 %
Interassay precision: 4.0 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 50 µl
Injection interval: 10 min
Fluorescence detector: 450 nm (exc.), 530 nm (em.)
HPLC-Thermostat: 40 °C

 

Sample Preparation

25000_flow

 

Chromatograms
ClinChek® Whole Blood Control, Level I ClinCheck® Whole Blood Control, Level II
25000_chrom1   25000_chrom2
Flavin adenine dinucleotide: 2.77 min, Flavin mononucleotide: 5.20 min, Riboflavin: 8.24 min   Flavin adenine dinucleotide: 2.77 min, Flavin mononucleotide: 5.18 min, Riboflavin: 8.26 min

 

Vitamin B2 in Whole Blood
Complete Kit, Order No. 25 000

Ordering Information

Order No. Description Quantity
25000  ClinRep® Complete Kit for Vitamin B2 in Whole Blood for 100 assays 1 pce. 
 
  Kit Components (order no. 25000):  
25010 Mobile Phase 1000 ml
25014 Test Solution 5 x 2 ml
25015 R Reconstitution Solution 10 ml
24513 Whole Blood Calibrator, lyophil. 5 x 2 ml
25020 Sample Preparation Vials 100 pcs.
25021 P Precipitant 30 ml
25022 S Stabilizing Reagent 50 ml
  Manual 1 pce.
  Quick Reference 1 pce.
     
  Start Accessories:  
27030 Analytical Column with test chromatogram 1 pce.
27032 Guard Column Holder incl. 1 Guard Column 1 pce.
 
  Accessories:  
27033 Guard Columns 5 pcs.
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
8874 Whole Blood Control, lyophil., Level I 10 x 2 ml
8875 Whole Blood Control, lyophil., Level II 10 x 2 ml
8876 Whole Blood Control, lyophil., Level I, II 2 x 5 x 2 ml


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