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Vitamin B1, B2 in Whole Blood and B6
in Plasma / Whole Blood (Combi Kit), Order No. 27000

 

The combi kit enables the combined measurement of the vitamins B1, B2, and B6. It consists of the chromatography kit (order no. 27000) and the particular sample preparation kits (order nos. 27001, 27002, 27006). The chromatography is performed with a single column for all B-vitamins in subsequent series of measurement. For switching the parameter (e.g. vitamin B1 --> vitamin B2) merely the parameter settings have to be changed (see parameter table below). By selection of the appropriate sample preparation kits, the customer is flexible to choose the application according to his needs.

  
  • Chromatography of 3 B-vitamins in 1 analytical series


  • Flexibility at changing demands for B1, B2 and B3 by
    1 Chromatography kit
    + 3 Sample preparation kits


Test data:
  Linearity:  
Vitamin B1: 1- 200 µg/l (1)  
Vitamin B2: 20 - 500 µg/l (2)  
Vitamin B6: 0.6 - 800 µg/l (3, 4), 0.6 - 1200 µg/l (5), 0.6 - 1500 µg/l (6)
     
  Detection limit: Determination limit:
Vitamin B1: 0.5 µg/l (1) 1 µg/l (1)
Vitamin B2: 10 µg/l (2) 20 µg/l (2)
Vitamin B6: 0.4 µg/l (3, 4, 5, 6) 0.6 µg/l (3, 4, 5, 6)
     
  Intraassay precision: Interassay precision:
Vitamin B1: 2.7 % (1) 4.2 % (1)
Vitamin B2: 3.8 % (2) 4.0 % (2)
Vitamin B6: 5.1 % (3), 2.5 % (4), 8.4 % (5), 2.3 % (6) 6.9 % (3), 3.1 % (4), 9.2 % (5), 2.9 % (6)
     
(1) determination as TPP, (2) determination as FAD, (3) determination as pyridoxal from plasma, (4) determination as PLP from plasma, (5) determination as pyridoxal from whole blood, (6) determination as PLP from whole blood


Parameter table for the combined determination of the B-vitamins:
       

 
Vitamin B1
Vitamin B2
Vitamin B6
Pump (Mobile Phase A + B):
flow rate 1 /ml/min
flow rate 1 /ml/min
flow rate 1 /ml/min
-Mixture of A : B:
ratio A : B = 93 : 7
ratio A : B = 80 : 20
ratio A : B = 100 : 0
Pump (Reagent N):
flow rate 0.1 ml / min
---
flow rate 0.1 ml / min
Injection volume:
50 µl
50 µl
50 µl
Injection interval:
6 min
10 min
10 min
HPLC-Thermostat:
35 ° C
40 ° C
25 ° C
Detector (exc. / em.)
376 / 435 nm
450 / 530 nm
370 / 470 nm
Retention times:
-Thiamine
approx. 5.1 min
---
---
-TPP
approx. 2.5 min
---
---
-Riboflavin
---
approx. 8.2 min
---
-FMN
---
approx. 5.2 min
---
-FAD
---
approx. 2.8 min
---
-Pyridoxal
---
---
approx. 8.8 min
-PLP
---
---
approx. 5.2 min

 

Sample Preparation:

See sample preparation of the respective complete kits:
Order no. 24000 (Vitamin B1), order no. 25000 (Vitamin B2), order no. 26000 (Vitamin B6)

 

Chromatograms:

see chromatograms of the respective complete kits:
Order no. 24000 (Vitamin B1), order no. 25000 (Vitamin B2), order no. 26000 (Vitamin B6)


Vitamin B1, B2 in Whole Blood and B6
in Plasma / Whole Blood (Combi Kit), Order No. 27000

Ordering Information
The combi kit consists of the chromatography kit (order no. 27000) and the separate
sample preparation kits (order nos. 27001, 27002, 27006).

A: Chromatography
Order No. Description Quantity
27000  ClinRep® Chromatography Kit for Vitamin B1, B2 and B6 for 1000 assays 1 pce. 
 
  Kit Components (order no. 27000):  
27008 Mobile Phase A 2400 ml
within the kit: 2 x order no. 27008
27009 Mobile Phase B 1000 ml
within the kit: 2 x order no. 27009
27030 Analytical Column with test chromatogram 1 pce.
27033 Guard Columns 5 pcs.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Accessories:  
27032 Guard Column Holder incl. 1 Guard Column 1 pce.
27033 Guard Columns 5 pcs.
 
  ClinChek® Controls:  
8870 Serum Control, lyophil., Level I 10 x 5 ml
8871 Serum Control, lyophil., Level II 10 x 5 ml
8872 Serum Control, lyophil., Level III 10 x 5 ml
8873 Serum Control, lyophil., Level I, II, III 3 x 3 x 5 ml
8874 Whole Blood Control, lyophil., Level I 10 x 2 ml
8875 Whole Blood Control, lyophil., Level II 10 x 2 ml
8876 Whole Blood Control, lyophil., Level I, II 2 x 5 x 2 ml



B: Sample Preparation
Order No. Description Quantity
27001  ClinRep® Sample Preparation Kit for Vitamin B1 in Whole Bloodfor 100 assays 1 pce. 
  Kit Components (order no. 27001):  
  Identical with order no. 24000*  
     
27002  ClinRep® Sample Preparation Kit for Vitamin B2 in Whole Blood for 100 assays 1 pce. 
  Kit Components (order no. 27002):  
  Identical with order no. 25000*  
     
27006  ClinRep® Sample Preparation Kit for Vitamin B6 in Plasma and Whole Blood for 100 assays 1 pce. 
  Kit Components (order no. 27006):  
  Identical with order no. 26000*  
 
* excepted the Mobile Phases, which are not included within the Sample Preparation Kits.


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