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Lamotrigine and Sulthiame in Plasma
Complete Kit, Order No. 15 100

 

Lamotrigine and sulthiame are important antiepileptic drugs (AED) in the management of several types of epilepsy. The ClinRep® complete kit is especially designed for the determination of lamotrigine and sulthiame from plasma and features in a short time for analysis (7 min) and an excellent test precision.

 
  • Robust and fast chromatography
    (injection interval: 7 min)
  • Excellent test precision


Test Data

  

HPLC Parameters
Linearity: 0.5 - 50 µg/ml
  (per analyte)
Recovery: 95 - 105 %
Lower detection limit: 0.2 µg/ml
  (per analyte)
Lower determination limit 0.5 µg/ml
  (per analyte)
Intraassay precision: 2.4 %
Interassay precision: 2.6 %
 
Pump: isocratic pump,
flow rate: 1.0 ml/min
Injection volume: 20 µl
Injection interval: 7 min*
UV/VIS-Detector: 265 nm
HPLC-Thermostate: 55°C
* In case the sample contains carbamazepine: 15 min.

 

Sample Preparation

15100_flow

 

Chromatograms
ClinChek® Plasma Control, Level I ClinCheck® Plasma Control, Level II
15100_chrom1   15100_chrom2
Sulthiame: 1.74 min, Lamotrigine: 2.44 min, IS: 4.64 min  
Sulthiame: 1.75 min, Lamotrigine: 2.45 min, IS: 4.64 min


Lamotrigine and Sulthiame in Plasma
Complete Kit, Order No. 15 100

Ordering Information

Order No. Description Quantity
15100  ClinRep® Complete Kit for Lamotrigine and Sultiame in Plasmafor 100 assays 1 pce. 
 
  Kit Components (order no. 15100):  
15010 Mobile Phase 1000 ml
15111 Standard Solution 3 ml
15112 P Precipitant with Internal Standard IS 15 ml
15113 Plasma Calibrator, lyophil. 3 ml
15020 Sample Preparation Vials with Reagent, lyophil. 50 pcs.
within the kit: 2 x order no. 15020
15021 D Diluting Solution 10 ml
  Manual 1 pce.
  Quick Reference 1 pce.
 
  Start Accessories:  
15030 Analytical Column with test chromatogram 1 pce.
     
  Refill Service:  
15031 Analytical Column with test chromatogram 1 pce.
  (only if you send back your original column)  
     
  Accessories:  
FK5810 PEEK tubing (connection of prefilter and column) 1 pce.
FK5820 Stainless steel prefilter holder 1 pce.
FK5821 Replacement filter for stainless steel prefilter holder 10 pcs.
 
  ClinChek® Controls:  
15180 Plasma Control for Lamotrigine and Sulthiame, lyophil., Level I 10 x 3 ml
15181 Plasma Control for Lamotrigine and Sulthiame, lyophil., Level II 10 x 3 ml
15182 Plasma Control for Lamotrigine and Sulthiame, lyophil., Level I, II 2 x 5 x 3 ml

 

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